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Alvedia’s scientific team established that DEA 1 antigen presents a large range of expression level on the RBC surface by using flow cytometer technology. Similarly to the Rhesus D molecule in human (named weak D or Du), some dogs express low level of DEA1 antigen (called weak DEA 1). This work shows that the weak DEA 1 were previously typed as DEA 1.2 positive dogs. A large study on more than 750 dogs shows that our membrane technology detects perfectly all range of DEA 1 antigen expression from strong to very weak. Consequently, the DEA 1 line will always show different intensities.
Example of DEA 1 blood typing results with different intensities using Flow cytometer analyser.
Example of DEA 1 blood typing positive results with different intensities using our labtest technology.
Flow cytometer analyser
Thanks to our specific membrane technology, the agglutinated red blood cells will be retained at the bottom of the membrane whereas non agglutinated rbc will carry on migrating until the top of the membrane.
In some rare cases, it can happen that the auto-agglutination is too strong to obtain a reliable result (migration).
For example: It can occur in case of cold agglutinin. (See Troubleshooting Procedure)
Thanks to the sensitivity of our specific monoclonal antibodies, even a low pcv will allow you to obtain a reliable blood typing.
DIRECT ANTIGLOBULIN TEST CLINICAL INFORMATION
A postive DAT is the corner stone to establish the IMHA diagnosis. In a dog suffering from anemia it is mandatory to perform a DAT as illustrated by the following cases.
CLINICAL CASE N°1
Name of patient: Vanda
Symptoms: Weakness, Lethargy, Pale Mucous membranes, Anorexia.
Biology: Anemia (PCV: 6,5%, Hb: 4,4g/L)
CLINICAL CASE N°2
Name of patient: Benji
Symptoms: Weakness, Lethargy, Small haematomas, Haematuria.
Biology: Anemia (PCV: 13,7%, Hb: 4,5g/L)
Cause: Haemostasis disorder
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THE TROUBLESHOOTING PROCEDURE
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