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IMPORTANCE OF DIRECT ANTIGLOBULIN TEST
Direct antiglobulin test (DAT) aims to detect the presence of immunoglobulin and/or C3 components on the surface of patient’s red blood cells (RBC).
DAT is commonly used in the diagnosis of immune mediated haemolytic anemia (IMHA) in dogs.
The classical technology uses incubation of washed RBC suspension with antisera that will cause agglutination.
Our immuno-chromatographic technology will detect the presence of immunoglobulin (IgG & IgM) and/or C3 components binding to the RBC surface. A positive result indicates an in vivo sensitization and can thus be used to indicate the presence of auto-immune antibodies.
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THE LAB TEST DAT PROCEDURE
DAT - : A negative reaction indicates absence of detectable antibodies or complement on the red cells.
DAT + : A positive reaction (+/- to ++++) indicates that the patient’s red cells are sensitized (red cells coated with antibodies and/or c3).
DAT + WEAK: A weak reaction attest of a positive result.
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1 BOX OF LAB TEST DAT CONTAINS
- 1 tube with 10 dat membranes (1 membrane = 1 dat)
- 1 dat buffer
- 1 wash buffer
- 10 tubes 5 ml
- 10 tubes 1,5 ml
- 10 blood collectors strips
- 10 small pipettes (1ml)
- 10 pipettes (3ml)
- 10 results forms
ADDITIONAL MATERIAL NEEDED
- A centrifuge
LAB TEST DAT PACKAGING
1 tube of Lab Test DAT = 10 membranes.
1 membrane = 1 determination
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